Small-sized primordial follicles were lost in the oocytes. Also, atretic follicles

Small-sized primordial follicles had been lost within the oocytes. Moreover, atretic follicles (Figure 5A) and regions of vacuolation (Figure 5B) were present. The integrity in the uterine tissue was not preserved (Figure 6A) plus the tunica albuginea layer was enlarged. In hUCSC, the ovary germinal epithelium was observed as mildly proliferated. Dominant follicles have been higher in quantity. Antral follicles had been surrounded with normal-sized preantral follicles (Figure 5C, D). Stromal vascularity as well as the quantity of endometrial glands were high. A higher quantity of cells within the connective tissue and also the superficial endometrium wasFigure 1. Pictures of your surgical process applied. a) Vertical incision in the abdominal wall. b) Placement of a sterile cannula in to the abdominal cavity for the application of talc powderFigure 2. Box plot in the primordial follicle counts in the groupsCNT: Handle group, hUCSC: Human umbilical cord mesenchymal stem cells (hUCMSCs)-applied group, AMN: Amniotic fluid (AF)applied group, hUCSC + AMN: Each hUCMSCs and AF-applied groupJ Turk Ger Gynecol Assoc 2022; 23: 154-Ganime Ayg and T entemur Stem cells and amniotic fluid in adhesionspresent. Epithelial gland cells were proportionate towards the surface epithelium, and also the glandular lumen was filled with secretion. The integrity in the uterine tissue was preserved (Figure 6B). In AMN, the ovary germinal epithelium was observed as proliferated. A high number of primordial follicles was present.A high number of corpus luteum was also present, suggesting ovulation (Figure 5E). Numerous endometrial glands and enhanced endometrial vascularity had been present (Figure 5F). The endometrium showed common proliferation (Figure 6C). In hUCSC + AMN, the ovary germinal epithelium was also observed as cubical-columnar cells, and the basal membraneFigure 3. Box plot on the quantity of endometrial glands with the groupsCNT: Handle group, hUCSC: Human umbilical cord mesenchymal stem cells (hUCMSCs)-applied group, AMN: Amniotic fluid (AF)applied group, hUCSC + AMN: Each hUCMSCs and AF-applied groupFigure 4. Box plot from the number of blood vessels with the groupsCNT: Handle group, hUCSC: Human umbilical cord mesenchymal stem cells (hUCMSCs)-applied group, AMN: Amniotic fluid (AF)applied group, hUCSC + AMN: Both hUCMSCs and AF-applied groupTable three. Comparison with the groups when it comes to uterus and ovarian histologyGroups ParametersPrimordial follicle count Preantral follicle count Quantity of endometrial glands Quantity of endometrial blood vesselsCNT (n=6) Imply SD Med. (min.-max.)50.8.three 51.five (37-64) three.67.9 3 (2-7) 7.67.two 7.5 (5-11) 0.67.8 0.five (0-2)hUCSC (n=6) Mean SD Med. (min.-max.)57.5.9 59 (47-66) 4.five.0 four (1-8) 13.Alkaline Phosphatase/ALPL Protein Source 05 11.EGF Protein Synonyms 5 (10-21) 1.PMID:24025603 33.eight 1.five (0-2)AMN (n=6) Mean SD Med. (min.-max.)97.8.6 99 (87-106) 7.two.7 eight (5-9) 23.eight.25 24.5 (19-27) 4.17.7 4 (3-5)hUCSC + AMN (n=6) Mean SD Med. (min.-max.)79.three.16 79.five (67-89) 5.8.9 5.5 (5-7) 19.8.4 11.8 (15-25) 3.0.six 3 (2-4)p0.001 0.055 0.001 0.CNT: Control group, hUCSC: Human umbilical cord mesenchymal stem cells (hUCMSCs)-applied group, AMN: Amniotic fluid (AF)-applied group, hUCSC+AMN: Both hUCMSCs and AF-applied group, SD: Normal deviation, Med.: Median, min.: Minimum, max.: Maximum. Kruskal-Wallis testTable 4. Post-hoc pairwise comparisons on the groups by Mann-Whitney U test.ParametersPrimordial follicle count Quantity of endometrial glands Number of endometrial blood vesselsCNT vs hUCSC0.310 0.009 0.CNT vs AMN0.002 0.002 0.CNT vs hUCSC hUCSC vs + AMN AMN0.002 0.002 0.00.