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Lau et al. BMC Complementary and Option Medicine 2013, 13:313 http:biomedcentral1472-688213RESEARCH ARTICLEOpen AccessNovel angiotensin I-converting enzyme JNK MedChemExpress inhibitory peptides derived from an edible mushroom, Pleurotus cystidiosus O.K. Miller identified by LC-MSMSChing Ching Lau1, Noorlidah Abdullah1 and Adawiyah Suriza Shuib1,AbstractBackground: Angiotensin I-converting enzyme (ACE) inhibitors have been reported to minimize mortality in individuals with hypertension. In comparison to chemosynthetic drugs, ACE inhibitors derived from organic sources such as meals proteins are believed to become safer for consumption and to have fewer adverse effects. Some edible mushrooms have been reported to substantially decrease blood pressure soon after oral administration. Furthermore, mushrooms are known to be wealthy in protein content material. This makes them a possible supply of ACE inhibitory peptides. Hence, the objective with the present study was to isolate and characterise ACE inhibitory peptides from an edible mushroom, Pleurotus cystidiosus. Solutions: ACE inhibitory proteins were isolated from P. cystidiosus depending on the bioassay guided purification steps, i.e. ammonium sulphate precipitation, reverse phase high overall performance liquid chromatography and size exclusion chromatography. Active fraction was then analysed by LC-MSMS and possible ACE inhibitory peptides identified were chemically synthesized. Impact of in vitro gastrointestinal digestions on the ACE inhibitory activity on the peptides and their inhibition patterns have been evaluated. Final results: Two prospective ACE inhibitory peptides, AHEPVK and GPSMR were identified from P. cystidiosus with molecular masses of 679.53 and 546.36 Da, respectively. Each peptides exhibited potentially higher ACE inhibitory activity with IC50 values of 62.eight and 277.five M, respectively. SEC chromatograms and BIOPEP analysis of these peptides revealed that the peptide sequence with the hexapeptide, AHEPVK, was steady throughout gastrointestinal digestion. The pentapeptide, GPSMR, was hydrolysed soon after digestion and it was predicted to release a dipeptide ACE inhibitor, GP, from its precursor. The Lineweaver-Burk plot of AHEPVK showed that this potent and steady ACE inhibitor has a competitive inhibitory impact against ACE. Conclusion: The present study indicated that the peptides from P. cystidiosus could possibly be possible ACE inhibitors. Though these peptides had reduce ACE inhibitor.