Tin tRNA (adenine-N1-)-methyltransferase activity response to carbohydrate stimulus tRNA (m1A) methyltransferase complicated protein homooligomerization clathrin binding nitrogen catabolite activation of transcription cell wall-bounded periplasmic space clathrin coat assembly protein localization to pre-autophagosomal RORγ Modulator Storage & Stability structure clathrin coat protein complex scaffold cAMP biosynthetic method adenylate cyclase activity adenylate cyclase-modulating G-protein coupled receptor signaling pathway cyclic nucleotide biosynthetic approach phosphorus-oxygen lyase activity mediator complex RNA polymerase II transcription cofactor activity regulation of transcription from RNA polymerase II promoter in response to zinc ion starvation sporulation resulting in formation of a cellular spore replicative cell aging zinc ion binding cellular response to methylmercury cellular response to amino acid starvation cellular response to glucose starvation cellular response to heat cellular response to ethanol cellular response to nitrosative stress cellular response to freezing cellular hypotonic response cellular hyperosmotic salinity response cellular response to alkalinity cellular response to acidity cellular response to hydrostatic stress cellular response to arsenic-containing substance cellular response to cold cellular response to hydrogen peroxide constructive regulation of transcription from RNA polymerase II promoter in response to strain age-dependent response to oxidative stress involved in chronological cell aging protein tyrosine kinase activity optimistic regulation of cytoplasmic mRNA processing physique assembly nucleic acid binding protein phosphatase type 1 complex translational initiation translation initiation issue activity helicase activity protein phosphatase regulator activity methylated histone residue binding regulation of ER to Golgi vesicle-mediated transport ribophagy regulation of retrograde vesicle-mediated transport, Golgi to ER ascospore-type prospore assembly 1-phosphatidylinositol-4-phosphate 5-kinase activity nucleosome positioning phosphatidylinositol phosphate kinase activity phosphatidylinositol metabolic course of action negative regulation of histone H3-K9 acetylation adverse regulation of DNA-dependent DNA replication negative regulation of histone H3-K14 acetylation chromatin DNA binding nucleolar chromatin termination of RNA polymerase II transcription termination of RNA polymerase I transcription protein serine/threonine/tyrosine kinase activity regulation of transcriptional get started website choice at RNA polymerase II promoterE15 7.5 0 -7.5 -15 15 7.5 0 -7.five -15 difference (P-value 0.05) 15 7.5 0 -7.five -15 15 7.5 0 -7.5 -15 15 7.5 0 -7.5 -15 15 7.five 0 -7.five -ClusterClusterRelative phosphorylation changeClusterClusterClusterClusterClusterClusterClusterClusterCluster1 2 3 four 5 six Cluster -1 0 1Position relative to the phosphorylated residueMembershipEnrichmentFIG. three. Dynamics of the rapamycin-regulated phosphoproteome. A, identification of considerably regulated phosphorylation sites. The histogram shows the distribution of phosphorylation web page SILAC ratios for 1h rapamycin/control (1h/ctrl) plus the distribution of unmodified peptide SILAC ratios (red). The cutoff for regulated phosphorylation websites was determined based on two common deviations from the median for unmodified peptides. Unregulated web sites are shown in black, and regulated internet sites are shown in blue. The numbers of down-regulated and NLRP1 Agonist Biological Activity up-regulated phosphorylation internet sites is indicate.