Ure 1B). Ultimately, a total of 15 coexpression modules have been constructed (Figure 1C). A total of 860 genes, accounting for 16.62 , were not assigned to any of these modules. We assigned a colour to and counted the amount of genes in every single module. There were 863 genes within the turquoise module, 788 within the blue module, 635 inside the brown module, 432 inside the yellow module, 382 within the green module, 270 inside the red module, 205 in the black module, 192 in the pink module, 179 inFrontiers in Veterinary Science | www.frontiersin.orgJuly 2021 | Volume 8 | ArticleLiu et al.Network for E. tenella Infected Chickenthe magenta module, 134 within the purple module, 76 within the greenyellow module, 47 within the tan module, 42 within the salmon module, 37 within the cyan module, and 33 within the midnight blue module.Infection Status Related Modules AnalysisTwo modules of yellow and magenta had been drastically related using the infection status (principal vs. secondary infection) by the module-trait evaluation (Figure 1E). For CDK2 web module yellow, genes had been involved in biosynthetic and metabolism method (nucleoside phosphate, organophosphate, and carbohydrate derivative) (Figure 3A) and metabolism VEGFR1/Flt-1 custom synthesis pathways (histidine, tyrosine, drug metabolism-cytochrome P450, tryptophan and phenylalanine) (Figure 3B). The expression amount of Genes in yellow module decreased considerably in the primary infection more than time and elevated inside the secondary infection more than time (Figure 3C). The idea networks showed the information of genes inside the prime five GO terms and KEGG pathways (Figures 3D,E). The coexpression network for module yellow genes was shown in Figure 3F. Genes in module magenta had been involved in immune response, defense response and actin filaments associated functions (Figure 4A). The expression amount of genes in this module elevated drastically in the key infection over time (Figure 4B). The idea networks showed the facts of genes within the prime 11 GO terms and IRF1, IFNG, and CAPZA1 had been circled because the significant genes (Figure 4C) and also identified as hub genes within this module (Figure 4D).Module Stability TestThe module stability showed that module pink, turquoise, blue, purple, green and black were among the most stable modules (connectivity correlation 0.eight). Module cyan displayed the least stability (Figure 1D).Evaluation of the Coexpression Module InteractionsWe analyzed the relationships between the 15 coexpression modules. Module eigengenes in this evaluation had been defined because the initial principal element of a coexpression module matrix. Cluster analysis was performed on these eigengenes (Figure 1E). The connectivity degree of eigengenes was determined to far better recognize the interactions in between the coexpression modules. The heatmap in Figure 1E showed the relatedness of your 15 coexpression modules identified by WGCNA, with red indicating close relatedness and blue indicating no relatedness. The outcomes demonstrated that the gene expression of every single module was mutually exclusive, indicating a higher degree of scale independence.Coexpression Modules Drastically Correlated With Diverse Infection StatusTo determine modules associated to main and secondary infection, we calculated the correlations amongst module eigengenes as well as the infection status (Figure 1F). The modules have been chosen applying a correlation p-value of 0.05 as a threshold. The genes inside the magenta (R = -0.61, p = 0.001) and yellow (R = 0.51, p = 0.01) modules are significantly positively or negatively correlated for the infection st.