The catalytic catalytic abilityas a substrate substrate the above the above final results. Three varieties

The catalytic catalytic abilityas a substrate substrate the above the above final results. Three varieties of the ability with N with N as a according to depending on benefits. 3 kinds of media (including LB, TB and M9) andand M9) and five substrate concentrations for this study for media (including LB, TB five substrate concentrations have been chosen have been chosen (Figure 5). The outcomes showed that the ideal substratethe ideal substrate 80 mg-1, and was L this study (Figure 5). The results showed that concentration was concentration the optimal L-1 , as well as the E Phospholipase A Formulation production wasfor E The highest was M9. The highest of E of 80 mgmedium for optimal medium M9. production conversion efficiency conversion the P2-carryingof E of was P2-carrying strain was 39.58 L-1), with a final substrate oncen- a efficiency strain the 39.58 three.6 (31.67 2.89 mg 3.6 (31.67 2.89 mg L-1 ), with tration of substrate concentration of 80 mg -1 inthat medium, followed by 2.52 mg edium L final 80 mg-1 in M9 medium, followed by M9 in TB medium (27.87 that in TB L-1), (27.87 in LB mg -1 although that in LB medium was theL-1). Essentially the most exciting -1 ). whilst that two.52 medium),was the lowest (22.72 1.14 mg owest (22.72 1.14 mgresult One of the most exciting outcome efficiency of E TLR7 list developed by the P2 3-carrying by the P2 3-carrying was that the conversion was that the conversion efficiency of E producedstrain in the constrain inside the conversion efficiency 2.85 mg-1). Therefore, M9 medium and M9 medium version efficiency was as much as (46.84 was up to (46.84 2.85 mg -1 ). Hence,80 mg-1 N and L L were80 mg -1 thewere chosen as theand substrate concentration, respectively, for the subchosen as N optimal medium optimal medium and substrate concentration, respectively, for study. sequent the subsequent study.Molecules 2021, 26, FOR Molecules 2021, 26, x 2919 PEER REVIEW8 13 8 ofofFigure Conversion efficiency of E in distinct media (LB, TB and M9) and substrate concentrations Figure five.5. Conversion efficiency of E in differentmedia (LB, TB and M9) and substrate concentra(substrate concentrations from 40 40 L-1L-1 120 mg – ). (a): the conversion efficiency of E of tions (substrate concentrations frommg gto to 120 mg1-1).(a): the conversion efficiency of E with the L the P2-carrying strain in LB, TB and M9 media. (b): the conversion efficiency of E with the P2 3P2-carrying strain in LB, TB and M9 media. (b): the conversion efficiency of E with the P2 3-carrying carryingin LB, TB and TB and M9 media. Information are as the indicates s.d.s s.d.s (n = three). strain strain in LB, M9 media. Information are shown shown because the means (n = 3).three.four. Substrate Diversity Analysis the HpaBC Complicated three.four. Substrate Diversity Evaluation ofof the HpaBC Complex To further investigate diversity of substrates, along with flavanone (N), a (N), To further investigate thethe diversity of substrates, along with flavanone mon- a monohydroxylated phenolic (p-coumaric acid, p-CA), dihydroflavonol (DHK), flavonol ohydroxylated phenolic acid acid (p-coumaric acid, p-CA), dihydroflavonol (DHK), flavonol (K), flavan-3-ol (afzelechin, Af) and anthocyanin (pelargonidin, PEL) were fed under the (K), flavan-3-ol (afzelechin, Af) and anthocyanin (pelargonidin, PEL) were fed beneath the optimal conditions, and the fermentation goods had been detected by HPLC and LC-MS optimal circumstances, and also the fermentation solutions were detected by HPLC and LC-MS solutions (Figure six). Prior studies have recommended that the HpaBC complex has in vivo strategies (Figure six). Preceding studies have.