By the Russian Foundation of Science (# 19-14-00110). Institutional Evaluation Board Statement: Not applicable. Informed

By the Russian Foundation of Science (# 19-14-00110). Institutional Evaluation Board Statement: Not applicable. Informed Consent Statement: Not applicable. Data Availability Statement: Not applicable. Conflicts of Interest: The authors declare that the analysis was performed in the absence of any commercial or financial relationships that might be construed as a potential conflict of interest.
H OH OHmetabolitesArticleDynamics with the Metabolome of Aliinostoc sp. PMC 882.14 in Response to Light and Temperature VariationsDamien Le Moigne 1 , Justine Demay 1,two , Anita Reinhardt 2 , C ile Bernard 1 , Sandra Kim Tiam 1,three and Benjamin Marie 1, UMR7245 Mol ules de Communication et Adaptation des Micro-Organismes (MCAM) MNHN-CNRS, Mus m National d’Histoire Naturelle, CP 39, 12 Rue Buffon, CEDEX 05, F-75231 Paris, France; [email protected] (D.L.M.); [email protected] (J.D.); [email protected] (C.B.); [email protected] (S.K.T.) Thermes de Balaruc-Les-Bains, 1 Rue du Mont Saint-Clair BP 45, 34540 Balaruc-Les-Bains, France; [email protected] UMR5557 Laboratoire d’Ecologie Microbienne, Universitde Lyon, 43 bd du 11 novembre 1918, Villeurbanne, F-69622 Lyon, France Correspondence: [email protected]; Tel.: 33-1-4079-Citation: Le Moigne, D.; Demay, J.; Reinhardt, A.; Bernard, C.; Kim Tiam, S.; Marie, B. Dynamics of your Metabolome of Aliinostoc sp. PMC 882.14 in Response to Light and Temperature Variations. Metabolites 2021, 11, 745. https://doi.org/ ten.3390/metabo11110745 Academic Editor: Daniel Globisch Received: 23 August 2021 Accepted: 26 October 2021 Published: 29 OctoberAbstract: Cyanobacteria are microorganisms able to adapt to a wide Aztreonam Protocol variety of environmental conditions and abiotic stresses. They produce a sizable variety of metabolites which will participate in the dynamic adaptation of cyanobacteria to a range of different light, temperature, and nutrient circumstances. Studying the metabolite profile is one way to realize how the physiological status of cells is related to their adaptive response. In this study, we sought to understand how the diversity and dynamics with the complete metabolome depended on the growth phase and several abiotic elements for example light intensity and temperature. The cyanobacterium, Aliinostoc sp. PMC 882.14, was selected for its substantial number of biosynthetic gene clusters. A single group of cells was grown under regular conditions as a manage, whilst other groups had been grown under greater light or temperature. Metabolomes had been analyzed by mass spectrometry (qTOF-MS/MS) combined with untargeted evaluation to investigate metabolite dynamics, and important variation was found in between exponential and stationary phases, no matter culture circumstances. Inside the higher light group, the synthesis of several metabolites, which includes shinorine, was induced when other metabolites, like microviridins, had been synthesized beneath larger temperature situations. Among 3-Chloro-5-hydroxybenzoic acid medchemexpress extremely regulated metabolites, we observed the presence of mycosporine-like amino acids (MAAs) and variants of somamides, microginins, and microviridins. This study demonstrated the importance of contemplating the physiological state of cyanobacteria for comparative international metabolomics and studies from the regulatory processes involved in production of particular metabolites. Our final results also open up new perspectives on the use of organisms such as cyanobacteria for the targeted production of bioactive metabolites. Keywords and phrases: cyanobacteria;.