The catalytic catalytic abilityas a substrate substrate the above the above final results. Three types of the capacity with N with N as a depending on based on outcomes. 3 sorts of media (which includes LB, TB and M9) andand M9) and five substrate concentrations for this study for media (including LB, TB five substrate concentrations were selected have been selected (Figure 5). The outcomes showed that the ideal substratethe ideal substrate 80 mg-1, and was L this study (Figure 5). The results showed that concentration was concentration the optimal L-1 , and also the E production wasfor E The highest was M9. The highest of E of 80 mgmedium for optimal medium M9. production ALK1 Inhibitor manufacturer conversion efficiency conversion the P2-carryingof E of was P2-carrying strain was 39.58 L-1), with a final substrate oncen- a efficiency strain the 39.58 three.6 (31.67 two.89 mg three.6 (31.67 2.89 mg L-1 ), with tration of substrate concentration of 80 mg -1 inthat medium, followed by two.52 mg edium L final 80 mg-1 in M9 medium, followed by M9 in TB medium (27.87 that in TB L-1), (27.87 in LB mg -1 whilst that in LB medium was theL-1). The most thrilling -1 ). even though that 2.52 medium),was the lowest (22.72 1.14 mg owest (22.72 1.14 mgresult Probably the most exciting result efficiency of E made by the P2 3-carrying by the P2 3-carrying was that the conversion was that the conversion efficiency of E producedstrain inside the constrain inside the conversion efficiency 2.85 mg-1). Therefore, M9 medium and M9 medium version efficiency was up to (46.84 was as much as (46.84 2.85 mg -1 ). Therefore,80 mg-1 N and L L were80 mg -1 thewere chosen as theand substrate concentration, respectively, for the subchosen as N optimal medium optimal medium and substrate concentration, respectively, for study. sequent the subsequent study.Molecules 2021, 26, FOR Molecules 2021, 26, x 2919 PEER REVIEW8 13 8 ofofFigure Conversion efficiency of E in different media (LB, TB and M9) and substrate concentrations Figure 5.5. Conversion efficiency of E in differentmedia (LB, TB and M9) and substrate concentra(substrate concentrations from 40 40 L-1L-1 120 mg – ). (a): the conversion efficiency of E of tions (substrate concentrations frommg gto to 120 mg1-1).(a): the conversion efficiency of E of your L the P2-carrying strain in LB, TB and M9 media. (b): the conversion efficiency of E on the P2 3P2-carrying strain in LB, TB and M9 media. (b): the conversion efficiency of E with the P2 3-carrying carryingin LB, TB and TB and M9 media. Information are because the means s.d.s s.d.s (n = 3). strain strain in LB, M9 media. Data are shown shown because the signifies (n = three).3.4. Substrate Diversity Evaluation the HpaBC Complicated three.four. Substrate Diversity Analysis ofof the HpaBC Complex To further Nav1.4 custom synthesis investigate diversity of substrates, along with flavanone (N), a (N), To additional investigate thethe diversity of substrates, along with flavanone mon- a monohydroxylated phenolic (p-coumaric acid, p-CA), dihydroflavonol (DHK), flavonol ohydroxylated phenolic acid acid (p-coumaric acid, p-CA), dihydroflavonol (DHK), flavonol (K), flavan-3-ol (afzelechin, Af) and anthocyanin (pelargonidin, PEL) had been fed below the (K), flavan-3-ol (afzelechin, Af) and anthocyanin (pelargonidin, PEL) were fed under the optimal conditions, plus the fermentation items were detected by HPLC and LC-MS optimal circumstances, as well as the fermentation items had been detected by HPLC and LC-MS solutions (Figure 6). Previous research have recommended that the HpaBC complex has in vivo procedures (Figure six). Prior research have.