Eed, the understanding of the mechanisms related to chemotherapy resistant is of good relevance. Strategies: A GAd cells line (AGS) was utilized to create a cell line resistant to 5-fluorouracil (rAGS_FU). Extracellular vesicles (EVs) secreted from AGS and rAGS_FU cell lines were isolated by ultracentrifugation, quantified and evaluated regarding their aggressiveness by means of invasion assays. Proteomics and Next generation sequencing evaluation of EVs and secreting cells had been also performed Outcomes: rAGS_FU cells secrete extra EVs and presented elevated invasion prices than AGS cells. Extracellular vesicles (EVs) derived from rAGS_FU cells had been capable to market resistance to chemotherapy and to induce an increase in invasion in AGS cells. Hence, cells resistant to chemotherapy have a more aggressive phenotype and are capable to transfer this acquired characteristics towards the non-resistant ones working with EVs. Proteomics analysis revealed that proteins involved in resistance to therapy which include FSCN1, are overexpressed or exclusively expressed in rAGS_FU EVs when compared toScientific Program ISEVAGS cells. Subsequent generation sequencing evaluation of the entire transcriptome (extended and short RNAs) from EVs and secreting cells revealed transcripts differentially expressed in between AGS and rAGS_FU cell lines including Melatonin Receptor Gene ID hsamiR-181a-5p and hsa-miR-372-3p which might be straight or indirectly, involved inside the resistant phenotype. Conclusion: A deep investigation of those information is needed to know and generate new possibilities for the discovery of new biomarkers of response to chemotherapy in gastric cancers and contribute towards the greater understanding on the biological part of molecules shuttled by EVs.PT04.Exosomal delivery of compact molecules for the management of ovarian cancer Farrukh Aqil1, Jeyaprakash Jeyabalan2, Radha Munagala1, Ashish Kumar Agrawal2, Lynne Parker3 and Ramesh C. Gupta1 Department of Aldose Reductase Inhibitor medchemexpress Medicine and JG Brown Cancer Center, University of Louisville, Louisville, KY, USA; 2JG Brown Cancer Center, University of Louisville, Louisville; 3Norton Healthcare Pavilion, Louisville, KY, USA; four Department of Pharmacology and Toxicology and JG Brown Cancer Center, University of Louisville, Louisville, KY, USAIntroduction: Ovarian cancer would be the fifth deadliest cancer among US women. Resistance to chemotherapy, lack of oral bioavailability and off-site toxicity of chemo drugs present important obstacles inside the treatment of sufferers with ovarian cancer. We hypothesised that drug molecules administered by means of exosomes will increase their oral bioavailability, and folic acid (FA)-functionalised exosomes will further boost therapeutic response and reduce off-target toxicities. Approaches: Exosomes (Exo) had been isolated from bovine milk and their size was measured by zetasizer. Small drug molecules (withaferin A (WFA), anthocyanidins (Anthos) and paclitaxel (PAC)) were loaded onto the Exo. Antiproliferative activity of Exo formulations was determined against ovarian cancer drug-sensitive (A2780) and drug-resistant (OVCA432) cells. Anti-tumour activity was determined against A2780 tumour xenografts in nude mice delivering the Exo formulations by oral gavage, except PAC which was offered i.p. Tumour targeting was achieved by co-loading in the tumour-targeting ligand, FA. Benefits: The isolated Exo showed the size of 93 8 nm. Test agents (WFA, Anthos and PAC) may be loaded onto Exo with 80 drug load. ExoWFA and ExoAnthos showed considerably larger (20 fold) antiproliferative activity v.