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For Children (MASC) is a comprehensive self-report instrument used for the assessment| Social Cognitive and Affective Neuroscience, 2016, Vol. 11, No.ABStrangerSubject*Best FriendFig. 1. Design of analysis parameters for best friend Cyberball. (A) A schematic design of the Quizartinib chemical information Cyberball set-up for participants. Each participant `played’ the game against two computerized players, one of they believed was their `Best Friend’ and the other they believed was a `Stranger’. The game began with a condition of fair play, where the ball could be passed between all players (as indicated by all the arrows), followed by a condition of exclusion, where the ball was passed between the computerized players (as indicated by arrows marked *). (B) Frontal left electrodes, white, were chosen to assess rejection-based ERPs.Authenticity of the game was enhanced with a GoogleTM page with a `Cyberball’ listing that was linked to a false loading screen. Participants were able to choose different gloves for play, different sound effects for throws and catches and different trajectories the balls were thrown in. Before the game started, the experimenter hinted with a verbal suggestion that `additional players’ were getting ready to play. Participants were debriefed about the falsity of the additional players and the game after the completion of the experiment. This version of Cyberball contained two conditions, 108 trials (throws) of fair play and 47 trials of exclusion. The game was fixed with a waiting period to receive the ball, waiting 0, 1, 2 or 3 trials before receiving it again (frequency 12, 12, 10 and 2, respectively). Immediately following fair play, the game transitioned into an exclusion phase. In this condition, there were 44 `rejection’ events where the ball was thrown between the other players and three `my turn’ events. This resulted in exclusion on 94 of the trials. For the purpose of the analysis, the three `my turn’ events, and the trial following each of these were excluded. Additionally the first 5 trials of the exclusion block were not used. Thus only 36 trials of rejection-based events were examined for analysis, divided into trials initiated by the `best friend’ and trials initiated by an unfamiliar child.channel cluster as in our previous Cyberball studies of frontal slow wave negativity (White et al., 2012; Sreekrishnan et al., 2014). EGI Hydrocell net channels 12, 18, 19, 20, 22, 23 and 24 were used for this analysis (Figure 1). ERP’s that corresponded to throws between the other players during exclusion were referred to as rejection-based ERPs. We further distinguished these throws between the other players (friend and stranger) during the exclusion phase. A throw by a friend to the stranger (as opposed to the participant) during exclusion was purchase CV205-502 hydrochloride considered a rejection-based ERP of friend. The throw by the stranger to the friend (as opposed to the participant) during exclusion was considered a rejection based ERP of stranger. The number of trials designated, as `rejection events’ were 36, 18 trials for rejection by friend and 18 trials for rejection by stranger. The mean number of trials available for averaging for Friend Rejection were Mean ?3.07; s.d. ?3.88 and Stranger Rejection were Mean ?11.67; s.d. ?3.77.AnalysesThe rejection based ERP’s were analyzed using SPSS v.19 software (SPSS Inc. Chicago, IL, USA). Because the data were collected as best friend dyads, we used a MIXED procedure was to account for the nesting of participants with.For Children (MASC) is a comprehensive self-report instrument used for the assessment| Social Cognitive and Affective Neuroscience, 2016, Vol. 11, No.ABStrangerSubject*Best FriendFig. 1. Design of analysis parameters for best friend Cyberball. (A) A schematic design of the Cyberball set-up for participants. Each participant `played’ the game against two computerized players, one of they believed was their `Best Friend’ and the other they believed was a `Stranger’. The game began with a condition of fair play, where the ball could be passed between all players (as indicated by all the arrows), followed by a condition of exclusion, where the ball was passed between the computerized players (as indicated by arrows marked *). (B) Frontal left electrodes, white, were chosen to assess rejection-based ERPs.Authenticity of the game was enhanced with a GoogleTM page with a `Cyberball’ listing that was linked to a false loading screen. Participants were able to choose different gloves for play, different sound effects for throws and catches and different trajectories the balls were thrown in. Before the game started, the experimenter hinted with a verbal suggestion that `additional players’ were getting ready to play. Participants were debriefed about the falsity of the additional players and the game after the completion of the experiment. This version of Cyberball contained two conditions, 108 trials (throws) of fair play and 47 trials of exclusion. The game was fixed with a waiting period to receive the ball, waiting 0, 1, 2 or 3 trials before receiving it again (frequency 12, 12, 10 and 2, respectively). Immediately following fair play, the game transitioned into an exclusion phase. In this condition, there were 44 `rejection’ events where the ball was thrown between the other players and three `my turn’ events. This resulted in exclusion on 94 of the trials. For the purpose of the analysis, the three `my turn’ events, and the trial following each of these were excluded. Additionally the first 5 trials of the exclusion block were not used. Thus only 36 trials of rejection-based events were examined for analysis, divided into trials initiated by the `best friend’ and trials initiated by an unfamiliar child.channel cluster as in our previous Cyberball studies of frontal slow wave negativity (White et al., 2012; Sreekrishnan et al., 2014). EGI Hydrocell net channels 12, 18, 19, 20, 22, 23 and 24 were used for this analysis (Figure 1). ERP’s that corresponded to throws between the other players during exclusion were referred to as rejection-based ERPs. We further distinguished these throws between the other players (friend and stranger) during the exclusion phase. A throw by a friend to the stranger (as opposed to the participant) during exclusion was considered a rejection-based ERP of friend. The throw by the stranger to the friend (as opposed to the participant) during exclusion was considered a rejection based ERP of stranger. The number of trials designated, as `rejection events’ were 36, 18 trials for rejection by friend and 18 trials for rejection by stranger. The mean number of trials available for averaging for Friend Rejection were Mean ?3.07; s.d. ?3.88 and Stranger Rejection were Mean ?11.67; s.d. ?3.77.AnalysesThe rejection based ERP’s were analyzed using SPSS v.19 software (SPSS Inc. Chicago, IL, USA). Because the data were collected as best friend dyads, we used a MIXED procedure was to account for the nesting of participants with.

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