Targeting mTOR with asTORi represents a potential new approach expression of 4EBP1 mRNA or protein

Since the expression of this helicase is relatively more frequent in higher grade tumors, it may serve as potential stage-specific marker for CC. Dosage alterations of these replication associated genes have vivid cytogenetic background. MCM2 which is over-expressed in cervical cancer irrespective of any clinical parameter is located. 3q21 shows high level of amplification in seven CC cell lines. Overall, 3q shows frequent copy number gains by comparative genomic hybridization in cervical cancer. Comprehensive cytogenetic approaches marked 8q as a region of high chromosomal gain in CC cell lines. Two of the replisome associated genes, MCM4 and Salidroside RECQL4 are included in this region. MCM4 has been detected as osteosarcoma driver gene as found to be over-represented in both copy number and expression profiles. In conclusion our study provides a comprehensive report of the expression profile of all the major MCM genes involved in human DNA replication and RECQL4, an important replisome associated factor in cervical cancer. Studies with larger sample size specifically of lower tumor stages can show significant correlation between expression levels of these genes and progressing tumor stages. This may give a better idea about the potentiality of these genes as stage specific markers. Further studies with precancerous lesions may provide clues as to whether these MCMs and RECQL4 can be therapeutic targets in cervical cancer. MicroRNAs nucleotides in length, are a major class of short endogenous non-coding RNA molecules that play important regulatory roles at the posttranscriptional level by targeting mRNAs for cleavage or translational repression. Since the discovery of miRNA molecules lin-4 and let-7 in 1993 in Caenorhabditis TAK-438 (free base) elegans through forward genetic screens, more and more novel miRNAs have been identified in almost all metazoan genomes, including worms, flies, plants and mammals by forward genetics, direct cloning, high-throughput sequencing technology and bioinformatics approaches. To date, 1600 miRNAs of the human genome have been annotated in the latest version of the miRBase. During the past several years, many methods have been proposed to compare the functional similarities between different protein-coding genes for further better understanding of the underlying biological phenomena or discovering previously unknown gene functions. With the growth of information on miRNAs, miRNAs have been shown as a group of important regulators to re