In addition a majority of these nuclei displayed an abundance of c-H2AX foci indicative

Additional protein interactions with 7-nAChRs and other nAChR subtypes have been reported by other groups that were not identified in this study. Our inability to detect these previously identified 7-nAChR-associated 331001-62-8 customer reviews proteins may reflect the ability of some proteins to compete with -bgtx binding, and thus be affected during the -bgtx affinity bead incubation. For example, the three-fingered toxin family member Lynx1 has been shown to interact competitively with -bgtx for binding to 7-nAChRs and was not identified in this study. This investigation expands upon our previous work from a murine model to a human model of Castanospermine 7-nAChR-associating proteins. The work described here is an example of how – bgtx-affinity may be harnessed as a tool for proteomic investigations of 7-nAChRs. Here we investigated receptor-protein interactions mediated by the differential expression of the Ric-3 chaperone. This approach can be applied to any protein to investigate possible alterations on the 7-nAChR interactome. Furthermore, this approach reproducibly identified a tryptic peptide of the 7-nAChR subunit. This peptide was identified in all SH-EP1-h7-Ric-3 and SH-EP1-h7 samples and was not observed in SH-EP1 samples. The size and reproducibility of this peptide could be used for absolute quantitation of 7-nAChRs by mass spectrometry using a heavy-labeled variant of the peptide. The study reported here presents a unique investigation of the role of Ric-3 in modification of the proteins associating with 7-nAChR. Identifying these proteins as members of the 7-nAChR macromolecular complex provides vital insight for understanding 7-nAChR surface expression and may assist in the identification of future therapeutic targets. Only Top and Co-Top protein identifications, i.e. only proteins identifications that can account for all peptide information within a protein group, were analyzed. For all 39 identified proteins, all Top and Co-Top identifications were either different isoform entries for protein products of the same gene or alternative database entries. Uniprot accession numbers, protein names, and gene names are provided for each Top and Co-Top entry. Also described per Top and Co-Top en