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Even so, no satisfactory method to figure out the efflux inhibitory routines of prospect compounds right is offered. Fluorescein-di-b-D-galactopyranoside is a fluorogenic compound that is non-64224-21-1 customer reviews fluorescent till it is hydrolyzed by b-galactosidase in the cytoplasm of Escherichia coli to generate a highly fluorescent dye, fluorescein. We initial verified that the two FDG and fluorescein are substrates of RND pumps in E. coli. In addition, current progress in microfabrication technologies including soft lithography has expanded their application in biology. In this study, we created a simple microfluidic channel unit for micro organism. By combining FDG and the microfluidic system, we developed a novel and highly sensitive method to consider the efflux inhibitory pursuits of compounds in opposition to P. aeruginosa MexB and MexY in E. coli, and clarified distinction of action mechanism among two inhibitors, APD597 pyridopyrimidine and PAbN.

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Author: calcimimeticagent